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| Registros recuperados: 12 | |
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| Spilki,Fernando Rosado; Franco,Ana Cláudia; Roehe,Paulo Michel. |
| Specific IgM, IgA, IgG1, IgG2, as well as neutralizing antibody responses were evaluated in sera of calves experimentally infected with two isolates of bovine herpesvirus type 1 (BoHV1) of distinct subtypes (subtype 1, BoHV1.1; subtype 2a, BoHV-1.2a). No significant differences were observed in the antibody responses induced by each BoHV-1 subtype. The antibody responses following primary acute infection were characterized by an increase in specific IgM and IgA levels between days 2 and 14 post inoculation (pi). IgG1 was detected from days 11 to 30 pi. IgG2 was detected on the sample taken on day 30 pi. Reactivation of infection following dexamethasone administration induced a significant rise in IgA levels, whereas IgG1 and IgG2 levels, which were at high... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine herpesvirus type 1; Infectious bovine rhinotracheitis; Immunoglobulin subclasses; ELISA. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200021 |
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| Hartmann,Tamahine Larronda Schmidt; Batista,Helena Beatriz de Carvalho Ruthner; Dezen,Diógenes; Spilki,Fernando Rosado; Franco,Ana Claudia; Roehe,Paulo Michel. |
| No presente estudo, foi realizada uma pesquisa em busca de anticorpos neutralizantes contra os vírus da cinomose (CDV) e da parainfluenza (CPIV) caninos em amostras de soro de 173 cães recolhidos a canis municipais em Novo Hamburgo (n=82) e Porto Alegre (n=91), RS. A pesquisa de anticorpos neutralizantes foi realizada pela técnica de soroneutralização frente a duas amostras vacinais de CDV (Rockborn e Snyder Hill) e frente a uma amostra de CPIV (V660). Em relação ao CDV, 95,9% das amostras de soros foram negativas para anticorpos neutralizantes contra a amostra Snyder Hill e 90,7% soronegativas para a amostra Rockborn. Entre os soropositivos (n=20; 11,6%), somente três deles apresentaram anticorpos neutralizantes frente às duas amostras de CDV testadas,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cinomose; Parainfluenza canino; Cães; Soroneutralização; Prevalência. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782007000400045 |
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| Kruger,Ernesto Renato; Penha,Tania Regina; Stoffelo,Daura Regina Eira; Roehe,Paulo Michel; Ribeiro,Magda Costa; Soccol,Vanete Thomaz. |
| Bovine Herpesvirus 4 (BoHV-4) is a member of Gammaherpesvirinaesub-family and belongs to genus Rhadinovirus. This virus has been associated with different clinical manifestations and research activity has put forward a strong correlation among virus infection, postpartum metritis, and abortion. The goal of this work was to characterize a virus strain isolate from a cow’s uterine outflow. From swabs drawn of uterine secretion, a virus strain was isolated and characterized by its cytopathology, morphology, and molecular biology approaches. In culture there was CPE development, characterized mainly by long strands with several small balloons along them, radiated from infected cells. Electron microscopy analysis revealed virus particles that had icosahedrical... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: BoHV-4 virus; PCR; RFLP; Fingerprint. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100279 |
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| Franco,Ana Cláudia; Hübner,Sílvia de Oliveira; Oliveira,Anna Paula de; Batista,Helena B. de C. Ruthner; Roehe,Paulo Michel; Rijsewijk,Franciscus A.M.. |
| Bovine herpesvirus 5 (BoHV-5) is a important cause of viral encephalitis in cattle in South America. Within the framework of developing a differential vaccine against BoHV-5, a deletion mutant was constructed based on a Brazilian BoHV-5 isolate. The target of the deletions were genes that code proteins implicated in the neurovirulence of BoHV-5, the glycoprotein I (gI), glycoprotein E (gE) and membrane protein US9. To construct the deletion mutant of BoHV-5, the flanking regions of all three genes were cloned in a prokaryotic plasmid. This deletion fragment was co-transfected with the viral DNA into bovine cells. Identification of deletion mutants was performed by immunostaining with an anti-gE monoclonal antibody. One of the gE negative viral populations... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine herpesvirus 5; BoHV-5; Deletion mutant; Glycoprotein genes. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400016 |
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| Spilki,Fernando Rosado; Luz,Roger Bordin da; Fabres,Rafael Bandeira; Soliman,Mayra Cristina; Kluge,Mariana; Fleck,Juliane Deise; Rodrigues,Manoela Tressoldi; Comerlato,Juliana; Cenci,Alexander; Cerva,Cristine; Dasso,Maurício Gautério; Roehe,Paulo Michel. |
| Viral gastroenteritis and other waterborne diseases are a major concern for health in Brazil. A number of studies were conducted about the presence of viruses on water samples from Brazilian areas. However, the knowledge about the occurrence of viral contamination of drinking water sources in rural settings of the country is insufficient. On the present work, 15 samples from 5 dairy farms located at the municipality of Tenente Portela were collected and analysed for the presence of human adenoviruses (HAdV), as well as human enteroviruses (EV) and rotaviruses (RV). HAdV was present on 66.66% of the water samples, and have been found in all samples from artesian wells and springs, which are used as sources of drinking water for the individuals inhabiting... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Human adenovirus; Water quality; Dairy farms. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300046 |
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| Lima,Francisco Esmaile de Sales; Batista,Helena Beatriz Ruthner; Campos,Fabrício Souza; Kunert Filho,Hiran Castagnino; Cibulski,Samuel; Finoketti,Fernando; Arantes,Thalita; Spilki,Fernando; Roehe,Paulo Michel; Rijsewijk,Franciscus Antonius Maria; Franco,Ana Cláudia. |
| This study aimed to analyze the effect of the expression of Parainfluenza virus 5 (PIV5) V protein in bovine cells on the replication of Bovine herpesvirus 5 (BoHV-5). Growth properties of BoHV-5 were evaluated in parental and PIV5 transfected cells. In one-step growth experiments, the BoHV-5 reached higher titers at earlier time points in the transfected cells when compared to the parental cells. The mean plaque size produced by the BoHV-5 in transfected cells was larger than the parental cells. This indicated that the expression of the PIV5 V gene facilitated the release and cell-to-cell spread of BoHV-5 in bovine cells. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: BoHV-5; V mRNA; Transfection. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100007 |
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| Riet-Correa,Franklin; Moojen,Valéria; Roehe,Paulo Michel; Weiblen,Rudi. |
| Revisam-se as doenças que devem ser consideradas no diagnóstico diferencial de febre aftosa. Dentre as doenças vesiculares ou erosivas, descrevem-se os principais aspectos relacionados ao diagnóstico da estomatite vesicular, diarréia viral bovina, febre catarral maligna, infecções por herpesvírus bovino 1 e 5, e uma estomatite ulcerativa associada a parvovírus bovino, que ocorreu no Rio Grande do Sul; língua azul, para a qual tem sido detectados anticorpos em bovinos e ovinos do Rio Grande do Sul; mamilite herpética que ocorre em outros Estados do País;peste bovina, que foi diagnosticada e erradicada no Estado de São Paulo em 1921; estomatite popular; e duas doenças exóticas:exantema vesicular e doença vesicular do suíno. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Febre aftosa; Doenças vesiculares; Estomatites ulcerativas; Herpesvírus bovino.. |
| Ano: 1996 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781996000200027 |
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| Registros recuperados: 12 | |
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